PCR · qPCR · RT-qPCR · ddPCR

PCR, qPCR, RT-qPCR & ddPCR

One resource for amplification-based assays — design, optimization, commercialization, and contract testing for preclinical and clinical studies anywhere in the world.

Discuss your assay Call 949-812-6902
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Amplification-based assays

From design to contract testing

Let us design, optimize, commercialize, or run your next PCR, qPCR, or ddPCR project. Our services support preclinical and clinical research, including multi-site studies.

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In-house platforms

qPCR

QuantStudio Dx, QuantStudio 5 Dx, QuantStudio Flex, QIAGEN Rotor-Gene Q MDx, Bio-Rad CFX96

Digital PCR

QuantStudio Absolute Q Digital PCR System

1

Design

Primers, probes, controls, and reaction formats for your target and specimen.

2

Optimize & validate

Performance tuned and validated to research, GLP, CLIA, or submission standards.

3

Commercialize

Transfer to kit form or launch as a test from our laboratory.

4

Contract testing

Sample testing for preclinical and clinical studies, including multi-site trials.

Applications

What we design, validate, and run

qPCR

Quantitative PCR

  • Gene expression
  • Copy number variation, including absolute CNV
  • Confirmatory testing
  • SNP genotyping and allelic discrimination
  • Methylation analysis (CpG)
  • Pathogen detection and quality control
  • Rare mutation detection
  • Low-abundance transcript detection
  • Viral titer (copies/mL) and vector copy number (copies/genome)
  • ctDNA and cfDNA detection in liquid biopsy
ddPCR

Digital PCR

  • Rare sequence and mutation detection
  • NGS pre- and post-run validation
  • Sensitive copy number variation detection
  • Viral titer (copies/mL)
  • Nucleic acid qualification
  • Single-cell analysis
  • Gene editing event detection
  • Gene expression and miRNA analysis
  • Liquid biopsy: cfDNA
  • Biodistribution, including gene therapy vectors

Choosing a method

qPCR or digital PCR?

qPCR

Real-time quantitative PCR

Quantification

Relative, or absolute against a standard curve

Best for

High throughput and a wide dynamic range, such as expression panels and routine testing

ddPCR

Digital PCR

Quantification

Absolute copy number, without a standard curve

Best for

Rare targets, small fold changes, and copy-number precision, such as ctDNA and vector biodistribution

Many programs use both: qPCR for screening and ddPCR to confirm low-level results.

Related

Assay Development

Liquid Biopsy

GxP & CLIA Testing

Testing Services

Let’s amplify your project.

Tell us about your target, specimen, and the quantification you need.

Discuss your assay Call 949-812-6902