EXO-NET® · Extracellular vesicles · Multi-omic analysis
Exosome isolation and analysis with EXO-NET®, offered through a licensing agreement with INOVIQ — from high-throughput extraction to RNA, DNA, and protein analysis.
What are exosomes?
Exosomes are membrane-bound extracellular vesicles (EVs), about 30–150 nm across, released by most cells and found in blood, saliva, urine, and cerebrospinal fluid.
EVs carry genetic material and proteins from their cell of origin. Those proteo-transcriptomic signatures can act as biomarkers — including for cancer — driving growing interest in EVs for diagnostics and therapeutics.
Isolation & analysis
EXO-NET is a fast, efficient, immunoaffinity magnetic bead‑based system for isolating exosomes from almost any biofluid. Its workflow suits high-throughput EV screening and exosome-based biomarker discovery for diagnostics and therapeutic targets.
EXO-NET performance
Comparison and characterization data for EXO-NET-isolated EVs.
From pooled normal human plasma, EXO-NET delivered equivalent or higher recovery of EV mRNAs and microRNAs (lower Ct values) than four other commercial EV isolation kits.
Western blot showed EVs positive for CD63, CD81, CD9, TSG101, and flotillin-1 and negative for calnexin; qPCR showed high yields of GAPDH mRNA and miR-191.
Western blot confirmed CD63, CD81, and CD9 with no detectable calnexin; qPCR confirmed high yields of GAPDH mRNA and U6.
By mass spectrometry, another bead-based kit showed twice the albumin peptide intensity and 6- to 10-fold lower EV peptide intensity than EXO-NET.
Data provided with EXO-NET. ResearchDx offers EXO-NET exosome isolation and analysis services under a licensing agreement with INOVIQ — view the agreement announcement (PDF).
Tell us about your sample type, volume, and the analytes you need.